psip1 novus (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
psip1 novus
Psip1 Novus, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psip1+novus/PSIP1+Antibody/pmc06138936-395-19-23
Average 92 stars, based on 8 article reviews
Psip1 Novus, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psip1+novus/PSIP1+Antibody/pmc06138936-395-19-23
Average 92 stars, based on 8 article reviews
psip1 novus - by Bioz Stars,
2026-09
92/100 stars
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1) Product Images from "Alternative splicing links histone modifications to stem cell fate decision"
Article Title: Alternative splicing links histone modifications to stem cell fate decision
Journal: Genome Biology
doi: 10.1186/s13059-018-1512-3
Figure Legend Snippet: Isoform switch from PBX1a and PBX1b during hESC differentiation. a Genome browser view shows the AS event and H3K36me3 signals of PBX1 upon hESC differentiation. The green horizontal bars below the ChIP-seq tracks indicate the narrow peaks called by MACS2. b The inclusion level for exon 7 of PBX1 is significantly correlated to the H3K36me3 signals over this exon across cell lineages. c The sequence difference of three protein isoforms of PBX1 and the main functional domains. d The relative expressions of PBX1a and PBX1b in 56 cells/tissues, representing the differential expressions of two isoforms in three groups based on their developmental states. e The expression levels of NANOG and OCT4 genes are negatively correlated with the expression of PBX1b. f The expression levels of PSIP1 and SRSF1 show significant positive correlations with the expression level of PBX1a. Also see Additional file : Figures S9, S10
Techniques Used: ChIP-sequencing, Sequencing, Functional Assay, Expressing
Figure Legend Snippet: Isoform switch of PBX1 links H3K36me3 to hESC fate decision. a qRT-PCR and western blot show the expression levels of Yamanaka factors in H1, MSC, and IMR90 cells. Whiskers denote the standard deviations of three replicates. b RT-PCR and western blot show the isoform switches between PBX1a and PBX1b from H1 cells to differentiated cells. c i. ChIP-PCR shows the differential binding of PBX1b to NANOG promoter in H1 cells and differentiated cells; ii. ChIP-PCR shows the reduced H3K36me3 signal in differentiated cells; iii. ChIP-PCR shows the differential recruitment of PSIP1 to exon 7 of PBX1. d RIP-PCR show the differential recruitment of SRSF1 around exon 7 of PBX1. e Co-IP shows the overall physical interaction between PSIP1 and SRSF1 in all studied cell types. f The mechanism by which H3K36me3 is linked to cell fate decision by regulating the isoform switch of PBX1, which functions upstream of the pluripotency regulatory network. Also see Additional file : Figures S9, S10
Techniques Used: Quantitative RT-PCR, Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction, Binding Assay, Co-Immunoprecipitation Assay
Related Articles
Chromatin Immunoprecipitation:Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein–DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell Signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein-DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Magnetic Beads:Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein–DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell Signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein-DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and ChIP-sequencing:Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein–DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell Signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein-DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Sequencing:Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein–DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell Signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein-DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Functional Assay:Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein–DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell Signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein-DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Expressing:Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein–DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell Signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein-DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Quantitative RT-PCR:Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein–DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell Signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein-DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Western Blot:Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein–DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell Signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein-DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Reverse Transcription Polymerase Chain Reaction:Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein–DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell Signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein-DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Binding Assay:Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein–DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell Signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein-DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Co-Immunoprecipitation Assay:Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein–DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell Signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and Article Title: Alternative splicing links histone modifications to stem cell fate decision Article Snippet: The protein-DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and |